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. 2021 Mar 10;14(8):1781–1804. doi: 10.1002/cssc.202100159

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© 2021 The Authors. ChemSusChem published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Scheme 15 — Artificially developed TaCo CO₂‐fixation cascade and its application in de novo synthesis. (a) TaCo‐based enzymatic cascade enabling conversion of glycolyl‐CoA to glycerate. ^[37] (b) Conversion of glycolate to glycerate using a TaCo based enzymatic cascade. (c). Conversion of ethylene glycol to glycerate in a 4‐step TaCo‐based enzymatic cascade. Enzymes: GCS=glycolyl‐CoA synthetase; GCC=glycolyl‐CoA carboxylase; TCR=tartronyl‐CoA reductase; GOx0313=alcohol dehydrogenase from Gluconobacter oxydans; PduP=aldehyde dehydrogenase from Rhodopseudomonas palustris BisB18.