FIGURE 2.

Footpad induration and isolated granuloma cells. A, B6, athymic nude, NOS2^−/−, and IFN‐γ^−/− mice were infected in the hind footpads with 30 × 10⁶ M. leprae, and induration was measured with a Vernier caliper. B, Cells were isolated from the footpad granulomas at 16 wk postinfection, and cytospin preparations were stained with a Diff‐Quik staining kit. B6 footpad granulomas contained macrophages and T cells. Athymic nude footpads are comprised of heavily infected macrophages (photograph originally published in ref. 103). T cells, macrophages, and multinucleated giant cells were recovered from NOS2^−/− footpads. IFN‐γ^−/− footpads contained large numbers of neutrophils