FIGURE 7.

Deletion of PGC‐1α in monocyte‐derived macrophages protects against pulmonary fibrosis. Ppargc1a^fl/fl or Ppargc1a^−/−Csf1r^MeriCreMer mice were administered tamoxifen (TAM) or regular (Reg) chow and exposed to saline or bleomycin (bleo). BAL cells were isolated 21 days later. A, mRNA analysis of PGC‐1α in BAL cells (n = 5‐6). B, TUNEL staining (n = 5‐6), and C, TUNEL quantification (n = 5‐6). Scale bar represents 50 μm. D, Tfam (n = 5), E, Cs (n = 5‐6), and F, Cox4i1 mRNA expression (n = 5‐6). G, mtDNA/nDNA in BAL cells (n = 4‐5). H, Masson’s trichrome staining of lung sections (n = 5‐6) and I, hydroxyproline analysis (n = 5). BAL cells were isolated from IPF patients by BAL. **P < .001; ***P < .0001. Values shown as mean ± S.E.M. One‐way ANOVA followed by Tukey’s multiple comparison test was utilized