Figure 5.

Real‐time enzyme kinetics by ¹⁹F NMR using F‐glycans. A) ¹⁹F NMR of F‐Lac incubated with β‐galactosidase. ¹⁹F NMR real‐time tracking of product formation (black arrows) upon incubation of F‐Lac with β‐galactosidase (right). Kinetic data were derived plotting the product formation rate as a function of the substrate concentration. The best fit of the experimental data provides a K _M value of 86.5±10.5 μm according to the Henry‐Michaelis–Menten equation (left). B) ¹⁹F NMR of F‐Lac incubated with Pmα23ST in presence of CMP‐Neu5Ac. The formation of F‐sLac (black arrows) can be followed by ¹⁹F NMR in real‐time. Product formation was confirmed by HPLC (Figure S9).