Figure 4.

MLG43‐triggered immunity in Arabidopsis is partially dependent on LysM‐PRRs CERK1, LYK4 and LYK5.

(a) Cross‐elicitation during the refractory period of Ca²⁺ signalling upon application of 50 μm MLG43, 50 μm chitohexaose and distilled water (mock). Data show the elevation of cytoplasmic Ca²⁺ concentration, measured as relative luminescence units (RLU), over time in 8‐day‐old Arabidopsis Col‐0^AEQ seedlings after treatments. Arrow indicates the application time of the second treatment within the refractory period of the first elicitation. Data represent mean ± σ (n = 8) in all panels.

(b) Mitogen‐activated protein kinases (MAPK) phosphorylation in 12‐day‐old Arabidopsis seedlings of Col‐0 plants and cerk1‐2, and lyk4 lyk5 mutants impaired in LysM‐PRR co‐receptors. Western blot using anti‐pTEpY antibody for phosphorylated MAPK moieties at different time points (5, 10 and 20 min). Black arrows indicate the position of phosphorylated MPK6 (top), MPK3 (middle) and MPK4/11 (bottom). Anti‐MPK6 and anti‐MPK3 were used as total protein control. Ponceau red‐stained membranes show equal loading. Chitohexaose (5 µm) and distilled water (mock) were used as controls. These results are from one representative experiment of the three performed that gave similar results.