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. 2021 Mar 22;106(3):601–615. doi: 10.1111/tpj.15185

Figure 5.

Figure 5

Mixed‐linked glucans are components of the cell walls of Hyaloperonospora arabidopsidis and trigger Arabidopsis disease resistance to this oomycete pathogen.

(a) β‐1,3/1,4‐glucan quantification in alcohol insoluble residues (AIRs) of H. arabidopsidis (Hpa isolate Noco2) conidiospores and of Arabidopsis Col‐0 plants non‐infected (mock‐treated) or infected with H. arabidopsidis (Noco2) 7 days post‐inoculation (dpi) with conidiospores (24‐day‐old Arabidopsis plants). Inoculated plants were extensively washed before AIR preparations in order to release H. arabidopsidis conidiospores from plant tissues. Data represent average ± σ (n = 3). Dashed line indicates the detection limit of the method used.

(b) Arabidopsis plants were foliar pre‐treated with MLG43 at two different concentrations 2 days before inoculation with H. arabidopsidis (Noco2). Presence of H. arabidopsidis in plants was quantified at 7 dpi (24 days old) as the abundance of conidiospores in inoculated per mg plant fresh weight. Data represent mean ± SE (n = 30). Statistically significant differences according to the Student's t‐test (*P < 0.05; **P < 0.01).