Figure 1.

The regeneration origin of cowpea EA explants via organogenesis.

(a) The structure of cowpea IT86D‐1010 EA extracted from imbibed mature seeds.

(b–d) The single (c) and multiple (d) shoot development from non‐decapitated EA explants with the plumule excised (b).

(e,f) The multiple shoot regeneration (f) from decapitated EA explants (e).

(g) Enlarged view of multiple shoot regeneration and the cutting site of the epicotyl indicated by the yellow arrow. The percentages in (c), (d) and (f) represent the rates of single and multiple shoot development determined from 100 non‐decapitated and 100 decapitated EA explants after 10 days on SIM. The red line through the middle of the epicotyl represents the decapitation process of EA explants to remove the SAM.