Table 4.
CRISPR/Cas9‐mediated gene editing efficiency and types for Vu‐SPO11 in IT86D‐1010
| Total # of T0 plants | # of T0 edited plants 1 | # of T0 edited plants with allele read percentage > 30% 2 | # of T0 edited plants with biallelic knockouts 3 | # of T0 edited plants with chimeric edits (≥2 edited alleles) | # of T0 edited plants with multiple‐bp deletion | # of edited T0 plants with 1‐bp G deletion | # edited T0 plants with 1‐bp G insertion |
|---|---|---|---|---|---|---|---|
| 35 | 24 (68.6%) 4 | 9 (25.7%) 4 | 2 (5.7%) 4 | 10 (41.6%) 5 | 8 (33.3%) 5 | 17 (70.8%) 5 | 9 (37.5%) 5 |
1
Edits are defined as mutagenesis at target site.
2
See Table S12 for the allele read percentage in each plant.
3
Biallelic knockout is defined as targeted mutagenesis that results in a frameshift mutation for both alleles.
4
The number in parentheses represents the frequency of the mutation type in the total T0 plants.
5
The number in parentheses represents the frequency of the mutation type in the total edited plants detected.