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. Author manuscript; available in PMC: 2022 Jul 23.
Published in final edited form as: Biochem Biophys Res Commun. 2021 May 28;563:31–39. doi: 10.1016/j.bbrc.2021.05.043

Figure 3. Pkp3-catenin associates with components of the canonical-Wnt destruction complex.

Figure 3.

(A) 293T cells in 100mm dishes were transfected with Flag-Pkp3 (8μg) and HA-GSK3beta (5 μg). The Pkp3:GSK3β association was resolved via anti-HA or anti-Flag immuno-precipitation/ immuno-blot. (B) Flag-Pkp3 (2μg) and Myc-Axin (2μg) were co-transfected in 293T cells. Immuno-precipitates (IP) of Flag-Pkp3 were immuno-blotted [19] with anti-Flag or -Myc antibodies to detect Pkp3 or Axin. (C) Flag-Pkp3 (3μg) and Myc-β-TrCP (3μg) were co-transfected into HaCaT cells. IPs were performed with anti-Myc antibody to precipitate β-TrCP, followed by immuno-blotting with anti-Flag antibody. (D) Endogenous Pkp3 coprecipitates with Myc-β-TrCP from HaCaT-cell extracts.