| Antibodies |
|
|
| Mouse monoclonal anti-nc82 |
University of Iowa Developmental Studies Hybridoma Bank |
RRID: AB_2314866 |
| Mouse monoclonal anti-GFP-20 |
Sigma-Aldrich |
RRID: AB_259941 |
| Alexa Fluor 488 goat polyclonal anti-mouse IgG |
Thermal Fisher |
RRID: AB_2576217 |
| Alexa Fluor 633 goat polyclonal anti-mouse IgG |
Thermal Fisher |
RRID: AB_2535719 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Normal Goat Serum |
Jackson ImmunoResearch Laboratories |
RRID: AB_2336990 |
| VECTASHIELD mounting medium |
Vector Laboratories Inc. |
REF#H-1000 |
| 10X phosphate bovine saline |
Sigma-Aldrich |
Cat#P5493 |
| Triton X-100 |
Sigma-Aldrich |
Cat#T8787 |
| Paraformaldehyde |
Electron Microscopy Sciences |
Cat#15710 |
| Collagenase |
Sigma-Aldrich |
Cat#C5138 |
| TexasRed dye |
Thermo-Fisher |
REF#D3328 |
| MgCl2 solution (1M in H2O) |
Sigma-Aldrich |
Cat#63069 |
| CaCl2 solution (1M in H2O) |
Sigma-Aldrich |
Cat#21115 |
| NaOH solution (10M in H2O) |
Sigma-Aldrich |
Cat#72068 |
| NaCl |
Sigma-Aldrich |
Cat#S7653 |
| KCl |
Sigma-Aldrich |
Cat#P5405 |
| HEPES |
Sigma-Aldrich |
Cat#H3375 |
| Trehalose |
Sigma-Aldrich |
Cat#T0167 |
| Sucrose |
Sigma-Aldrich |
Cat#S1888 |
| NaHCO3
|
Sigma-Aldrich |
Cat#S5761 |
| NaH2PO4
|
Sigma-Aldrich |
Cat#S5011 |
| Experimental Models: Organisms/Strains |
|
|
|
D. melanogaster: yw;N-Synaptobrevin-GAL42.1;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;;R11F07-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 39414 |
|
D. melanogaster: y1w1118;;R11F08-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48467 |
|
D. melanogaster: y1w1118;;R12C04-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48494 |
|
D. melanogaster: y1w1118;;R19H07-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48867 |
|
D. melanogaster: y1w1118;;R20G07-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48613 |
|
D. melanogaster: y1w1118;;R30E11-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48100 |
|
D. melanogaster: y1w1118;;R31C03-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 48103 |
|
D. melanogaster: y1w1118;;R53B06-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 38863 |
|
D. melanogaster: y1w1118;;R44H11-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 41268 |
|
D. melanogaster: y1w1118;;R72D07-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 39770 |
|
D. melanogaster: y1w1118;;R91H11-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 40596 |
|
D. melanogaster: y1w1118;;R95F09-GAL4attP2; |
Bloomington Drosophila Stock Center |
BDSC: 40712 |
| D. melanogaster: y1w1118;;R13F02-GAL4ADVK00027,R85D07-GAL4DBDattp2; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;R52H09-GAL4ADattP40;R18F09-GAL4DBDattP2; |
Bloomington Drosophila Stock Center |
BDSC: 68267 |
|
D. melanogaster: y1w1118;;R19H07-GAL4ADVK00027;R20G07-GAL4DBDattP2; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;UAS-C3PA-GFPunknown;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;UAS-C3PA-GFPattP40;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;;UAS-C3PA-GFPattP2; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;;UAS-C3PA-GFPVK00005; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: y1w1118;UAS-SPA-GFPattP40;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;UAS-syb::spGFP1-10unknown;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;UAS-DenMark2;; |
Bloomington Drosophila Stock Center |
BDSC: 33062 |
|
D. melanogaster: yw;; UAS-synaptotagmin::GFP3; |
Bloomington Drosophila Stock Center |
BDSC: 6926 |
|
D. melanogaster: yw;UAS-tdTomatoattP40;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;MB247-GAL80unknown;; |
Bloomington Drosophila Stock Center |
BDSC: 64306 |
|
D. melanogaster: yw;;MB247-LEXA::VP16unknown; |
Pitman et al., 2011 |
N/A |
|
D. melanogaster: yw;LEXAop-CD4::spGFP11unknown;; |
Macpherson et al., 2015 |
N/A |
|
D. melanogaster: yw;LEXAop-tdTomatoSu(Hw)attP5;; |
Bloomington Drosophila Stock Center |
BDSC: 56142 |
|
D. melanogaster: yw;;N-Synaptobrevin-QF; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;QUAS-C3PAunknown;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;QUAS-SPAunknown;; |
Aso et al., 2014 |
N/A |
|
D. melanogaster: yw;MB247-DsRedunknown;; |
Riemensperger et al., 2005 |
N/A |
| Software and Algorithms |
|
|
| ImageJ |
Schindelin et al., 2012 |
https://imagej.nih.gov/ij/ |
| Prairie View |
Bruker |
|
| ZEN microscope software |
ZEISS |
|
| Other |
|
|
| FlyLight |
Jenett et al., 2012 |
http://www.janelia.org/project-team/flylight |
| Virtual Fly Brain |
Jenett et al., 2012 |
https://v2.virtualflybrain.org |
| NeuPrint web interface for the hemibrain connectome database |
Xu et al., 2020 |
https://neuprint.janelia.org |
| Borosilicate glass with filament |
Sutter Instruments |
ITEM#BF100-50-10 |
