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. 2021 Jun 18;11:656608. doi: 10.3389/fonc.2021.656608

Figure 1.

Figure 1

Expression of Rac1, Cdc42, p-mTOR, and autophagy- and apoptosis-related molecules in RMS tissues. (A, B) Quantitative real-time PCR (qRT-PCR) was used to detect the mRNA expression levels of Rac1 (A) and Cdc42 (B) in 48 RMS cases and 13 normal muscle tissue cases. (C, D) HE and IHC staining of Rac1 (C) and Cdc42 (D) in normal and RMS tissues. (E) Kaplan–Meier analysis and log-rank test were applied to determine the relationship between Rac1 protein expression and patient survival. (F) HE and IHC staining of p-mTOR in normal and RMS tissues. (G) IHC staining of Beclin1 and LC3 in normal and RMS tissues. (H) IHC staining of Bax and Bcl-2 in normal and RMS tissues. A representative image is provided. ***P < 0.001.