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. 2021 Jun 18;11:656608. doi: 10.3389/fonc.2021.656608

Figure 3.

Figure 3

GEFT can inhibit autophagy and apoptosis in RMS cells via Rac1. (A) Western blotting was used to detect the expression of Beclin1 and LC3 protein in RH30 cells transfected with GEFT, GEFT+NSC23766, GEFT+CQ, and GEFT+CQ+NSC23766. (B) Western blotting was used to detect the expression of Beclin1 and LC3 protein in RD cells transfected with GEFT, GEFT+NSC23766, GEFT+BafA-1, and GEFT+BafA-1+NSC23766. (C) The representative images of RH30 and RD cells transfected with GEFT and GEFT+NSC2376 were detected by immunofluorescence, MDC and AO staining. (D) The representative images of RH30 and RD cells transfected with GEFT and GEFT+NSC2376 were detected by TUNEL staining. (E) Western blotting was used to detect the expression of GEFT, Total Rac1, Active Rac1, Bax, Bcl-2, Caspase-3, and Cleaved-PARP protein in RH30 and RD cells transfected with GEFT and GEFT+NSC23766. (F) Flow cytometry was used to detect the apoptosis of RH30 cells and GEFT-transfected RH30 cells in GEFT, GEFT+CQ, GEFT+NSC23766 and GEFT+NSC23766+CQ groups. Flow cytometry was used to detect the apoptosis of RD cells and GEFT-transfected RD cells in GEFT, GEFT+BafA-1, GEFT+NSC23766 and GEFT+NSC23766+BafA-1 groups. ***P < 0.001, *P < 0.05.