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. 2021 Jun 18;11:656608. doi: 10.3389/fonc.2021.656608

Figure 4.

Figure 4

GEFT can inhibit autophagy and apoptosis in RMS cells via Cdc42. (A) Western blotting was used to detect the expression of Beclin1 and LC3 protein in RH30 cells transfected with GEFT, GEFT+ZCL278, GEFT+CQ, and GEFT+CQ+ZCL278. (B) Western blotting was used to detect the expression of Beclin1 and LC3 protein in RD cells transfected with GEFT, GEFT+ZCL278, GEFT+BafA-1, and GEFT+BafA-1+ZCL278. (C) The representative images of RH30 and RD cells transfected with GEFT and GEFT+ZCL278 were detected by immunofluorescence, MDC and AO staining. (D) The apoptosis of RH30 and RD cells transfected with GEFT and GEFT+ZCL278 was detected by TUNEL. (E) Western blotting was used to detect the expression of GEFT, Total Cdc42, Active Cdc42, Bax, Bcl-2, Caspase-3, and Cleaved-PARP protein in RH30 and RD cells transfected with GEFT and GEFT+ZCL278. (F) Flow cytometry was used to detect the apoptosis of RH30 cells and GEFT-transfected RH30 cells in GEFT, GEFT+CQ, GEFT+ZCL278 and GEFT+ZCL278+CQ groups. Flow cytometry was used to detect the apoptosis of RD cells and GEFT-transfected RD cells in GEFT, GEFT+BafA-1, GEFT+ZCL278 and GEFT+ZCL278+BafA-1 groups. ***P < 0.001, *P < 0.05.