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mTOR can be regulated by Rac1/Cdc42. (A, B) The Rac1 (A) and Cdc42 (B) inhibitors were added to the RMS cells. p-mTOR and mTOR were detected by Western blot, and β-actin was used as a control. (C) The Rac1 and Cdc42 antibodies were used to pull down mTOR by using the co-immunoprecipitation assay. (D, E). Rac1 (D) and Cdc42 (E) were pulled down using the mTOR antibody, and the results were examined by Western blot. ***P < 0.001, **P < 0.01, *P < 0.05.
