Figure 3.

Possible algorithm for searching for residual leukemic cells in patients with B-cell precursor acute lymphoblastic leukemia after CD19-targeted therapy. Various ways of B-lineage gating are shown with their limitations indicated. Consecutive investigation of all these B-cell compartments together with the CD45-defined blast region, precursor regions and aberrantly expressed antigens can help to overcome changes in expression of CD19 and other antigens, applicable for minimal residual disease monitoring.