Figure 6.
Matrix metalloproteinases are upregulated in bone marrow-derived macrophages with JAK2 V617F expression. (A) Mononuclear cells isolated from bonemarrow (BM) cells of wild-type (WT) mice or JAK2V617F mice were cultured in the presence of granulocyte-macrophage colony stimulating factor for 6 days. Representative immunofluorescence images of the cells stained with anti-CD68 (red) antibody and DAPI (blue). Scale bars, 100 m. (B) Relative mRNA expression levels of Mmp2, Mmp9, and Mmp13 in the cultured mononuclear cells (n=7 each). (C) Mmp2 and Mmp9 mRNA expressions with and without ruxolitinib pretreatment at 250 nM for 24 hours in the JAK2V617F mononuclear cells (n=4 each). Dimethylsulfoxide (DMSO) was used for control. Actb was used for normalization. The average value for the cultured mononuclear cells from WT mice or DMSO group was set to 1. All data are presented as mean ± standard error of the mean. *P<0.05 vs. the WT group or DMSO group by the unpaired Student’s t-test. JAK2V617F: JAK2 V617F-expressing transgenic mice.