Figure 2.

Clinical significance of CD3⁺ T-cells and CD163⁺ TAMs density in HGSC. Univariable overall survival estimates (Kaplan–Meier method) according to CD3⁺ T-cells (A) and CD163⁺ TAMs (B) densities; p-values estimated with log-rank test. Treemap showing subgroup composition based on immunoscore and results of association analysis between immunoscore and chemotherapy response (CHT resp.), platinum resistance (Plat. Res.) and survival events (Death or Recurrence) (C). Heatmap of unsupervised hierarchical clustering by Euclidean distance of log₂CD3⁺ T-cells and log₂CD163⁺ TAMs densities, each row represents a patient (D). Univariable overall survival estimate (Kaplan–Meier method) of immunoscore classes (E), pairwise comparisons p-values adjusted with FDR. Forest-plot of multivariable overall survival analysis (F). Cut-offs for CD3^Hi and CD163^Hi densities were set at median values for each distribution. *P < 0.05; **P < 0.01.