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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Curr Res Biotechnol. 2020 Feb 18;2:16–21. doi: 10.1016/j.crbiot.2020.02.002

Table 1.

Biotinylated bioconjugate synthesis and peptide quantification

WYR Addition
Molecule WYR added (mols per CS) Absorbance@280 nm Peptide Bound (mols per CS) Coupling Efficiency CoF (kinetic) CoF (static)
2GAH_13WYR_CS 16.5 2.1 13.2 80% 0.19±0.04 0.22±0.02
5WYR_CS 5.5 1.1 5.5 100% 0.30±0.01 0.26±0.03
9WYR_CS 11 1.6 9.0 82% 0..26±0.05 0.23±0.03
15WYR_CS 16.5 2.2 14.6 89% 0.19±0.04 0.22±0.03
GAH Addition
Molecule GAH added (mols per CS) Absorbance@280 nm Peptide Bound (mols per CS) Coupling Efficiency CoF (kinetic) CoF (static)
2GAH_13WYR_CS 2.4 0.4 2.0 84% 0.19±0.04 0.22±0.02
2GAH_CS 2.4 0.4 2.0 84% 0.31±0.04 0.27±0.03
4GAH_CS 5.5 0.7 4.0 73% 0.31±0.02 0.26±0.02
9GAH_CS 11 1.3 9.0 82% 0.27±0.02 0.23±0.02
12GAH_CS 16.5 1.7 12.3 75% 0.28±0.03 0.26±0.02