Fig. 2.

LINC00261 is required for NEPC cell viability and invasiveness. (A) Expression (qPCR) of LINC00261 in a panel of prostate cell lines. (B) Subcellular localization of LINC00261, GAPDH, and MALAT1 RNA transcripts in PC‐3 cells. (C) Viability (MTT assay) of PC‐3 cells treated with a control, nontargeting siRNA (siNC), or distinct siRNAs targeting LINC00261 at 10 nm dosage. (D) Cell viability‐adjusted activity of caspase 3/7 after 72 h of siRNA‐mediated LINC00261 knockdown in PC‐3 cells. (E) Left, Representative images of iodonitrotetrazolium chloride stained soft agar PC‐3 cell colonies after treatment with either the nontargeting, control siRNA (siNC), or siRNA targeting the LINC00261 (siLINC00261) transcript (two‐tailed t‐test). Right, Average number of soft agar colonies per 10× field of siNC‐ or siLINC00261‐treated PC‐3 cells. The scale bar at the bottom marks a 10 μm width. Distinct cells colonies were counted from three randomly chosen 10X field per well from two biological replicates. All data are presented as mean ± SEM with at least two independent biological replicates. (C) Analyzed using two‐way ANOVA with Tukey's multiple comparisons test. (D) Analyzed by one‐way ANOVA with Dunnett's post hoc test. (E) Analyzed by two‐tailed t‐test. *P < 0.05; ****P < 0.001.