Figure 6.

Immunosuppressive phenotype exists in high-risk tumors. (a) Results of ESTIMATE score and differentially infiltrated immune cells evaluated by CIBERSORT and IMMUNECELL AI between high- and low-risk group in TCGA. (b) Relative cell abundance of Macrophage and Treg calculated by CIBERSORT and IMMUNECELL AI between two groups in TCGA. (c) Cell percentage of CD68+ Macrophage, CD68+ CD163+ Macrophage M2, FOXP3+ Treg and PD-L1 expression in tumors detected by mIHC in the TMA cohort. (d) Representative immunofluorescence staining pictures of high- and low-risk tumors. DAPI (blue), CD68 (purple), CD163 (red), FOXP3 (green). (e) Differentially expressed genes profile involved in the negative regulation of the Cancer-Immunity Cycle between high- and low-risk group. (f) Expression of the common immune checkpoints between high- and low-risk group. (g) Expression of the immune suppressive cytokines between high- and low-risk group. *P < .05.**P < .01.***P < .001

mIHC, multiplex immunohistochemistry; TCGA, The Cancer Genome Atlas; TMA, tissue microarray.