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. 2021 Jun 30;12(1):382–395. doi: 10.1080/21645698.2021.1944013

Figure 1.

Figure 1.

Restriction analysis of puc57 vectors containing the cry1Fa and cry32Aa gene cassette (total 7954 bp size) or the AtPME and AnPME gene cassette (total 7893 bp size). Figure 1a, BamHI and EcoRI restriction analysis of the cry1Fa and cry32Aa gene cassette. M: 10kb ladder. Lane 2: cry1Fa and cry32Aa gene cassette positive sample. Lane 1: negative control of pUC57 without insert samples. Figure 1b, EcoRI and HindIII restriction analysis of the AtPME and AnPME gene cassette. M: 10 Kb ladder. Lane 1, 2: AtPME and AnPME gene cassette positive samples. Figure 1c, schematic representation of both gene cassettes containing cry1Fa and cry32Aa genes or AtPME and AnPME genes, respectively