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Cellular uptake of nR-NLC by clearage method. (A) The cellular uptake of Cou 6-loaded nR-NLC by A549 cells at different incubation time at 37 °C, the Cou 6 concentration was at 2 μg/ml. (B) The cellular uptake of Cou6-loaded nR-NLC at 37 °Cand 4 °C at 4 h, the Cou6 concentration was at 2 μg/ml. Results are presented as mean ± SD (n = 3). Significant differences from 0R-NLC are indicated as follows: *p < 0.05, **p < 0.01 and ***p < 0.001.
