Table 1. Composition of transfersomal formulations and observed responses in Box–Behnken design for transfersomal formulations containing EGCG and HA.
| Batches | SPC (mg) | SC (mg) | EGCG (mg) | HA (mg) | Sizea (nm) | Zeta potentiala (mV) | EE of EGCGa (%) | EE of HAb (%) |
|---|---|---|---|---|---|---|---|---|
| ETF1 | 85 | 5 | 5 | 1.75 | 101.7 ± 4.16 | −60.7 ± 3.04 | 66.31 ± 1.76 | 30.68 ± 3.04 |
| ETF2 | 85 | 15 | 15 | 3 | 176.7 ± 8.20 | −52.9 ± 4.14 | 64.10 ± 1.43 | 35.70 ± 3.85 |
| ETF3 | 95 | 15 | 10 | 0.5 | 91.7 ± 5.13 | −36.3 ± 1.14 | 64.69 ± 2.98 | 15.00 ± 1.56 |
| ETF4 | 85 | 25 | 10 | 3 | 105.7 ± 6.29 | −46.5 ± 2.94 | 49.36 ± 2.60 | 21.85 ± 2.14 |
| ETF5 | 85 | 15 | 5 | 0.5 | 103.7 ± 7.53 | −52.0 ± 3.87 | 57.63 ± 2.24 | 12.00 ± 1.30 |
| ETF6 | 85 | 25 | 15 | 1.75 | 206.8 ± 12.03 | −48.8 ± 3.56 | 54.89 ± 1.78 | 21.60 ± 1.90 |
| ETF7 | 85 | 15 | 10 | 1.75 | 105.7 ± 4.40 | −43.5 ± 5.32 | 73.08 ± 2.34 | 43.14 ± 4.23 |
| ETF8 | 95 | 25 | 10 | 1.75 | 122.7 ± 7.16 | −45.7 ± 4.59 | 46.84 ± 1.56 | 18.45 ± 1.34 |
| ETF9 | 75 | 15 | 10 | 0.5 | 131.3 ± 6.38 | −44.1 ± 4.32 | 73.57 ± 1.67 | 57.00 ± 4.98 |
| ETF10 | 75 | 25 | 10 | 1.75 | 109.6 ± 5.27 | −53.3 ± 4.34 | 74.42 ± 2.45 | 36.57 ± 2.15 |
| ETF11 | 95 | 5 | 10 | 1.75 | 112.8 ± 6.42 | −45.1 ± 4.35 | 51.88 ± 1.15 | 17.48 ± 2.19 |
| ETF12 | 85 | 5 | 10 | 0.5 | 112.1 ± 5.50 | −41.2 ± 3.89 | 62.21 ± 4.40 | 19.56 ± 2.20 |
| ETF13 | 95 | 15 | 15 | 1.75 | 234.6 ± 8.71 | −45.1 ± 4.10 | 43.30 ± 2.76 | 21.70 ± 1.28 |
| ETF14 | 85 | 15 | 10 | 1.75 | 107.4 ± 5.98 | −47.2 ± 4.45 | 73.22 ± 2.37 | 46.20 ± 4.97 |
| ETF15 | 75 | 15 | 10 | 3 | 164.0 ± 8.27 | −45.6 ± 3.82 | 46.10 ± 1.16 | 27.73 ± 3.20 |
| ETF16 | 75 | 15 | 5 | 1.75 | 96.8 ± 3.44 | −39.9 ± 3.75 | 72.63 ± 2.89 | 32.85 ± 3.10 |
| ETF17 | 85 | 15 | 5 | 3 | 132.8 ± 5.03 | −48.9 ± 4.26 | 67.36 ± 1.65 | 23.04 ± 3.56 |
| ETF18 | 85 | 5 | 15 | 1.75 | 187.5 ± 9.66 | −47.8 ± 4.98 | 63.20 ± 2.23 | 27.98 ± 3.56 |
| ETF19 | 85 | 15 | 10 | 1.75 | 103.3 ± 5.34 | −41.6 ± 4.38 | 75.00 ± 1.45 | 46.00 ± 2.60 |
| ETF20 | 85 | 15 | 10 | 1.75 | 101.2 ± 4.82 | −44.8 ± 4.24 | 76.53 ± 2.68 | 48.57 ± 4.35 |
| ETF21 | 85 | 15 | 15 | 0.5 | 145.2 ± 8.25 | −43.2 ± 3.80 | 49.50 ± 3.50 | 19.20 ± 2.39 |
| ETF22 | 85 | 25 | 5 | 1.75 | 93.55 ± 4.73 | −30.6 ± 3.69 | 54.73 ± 3.92 | 53.14 ± 4.78 |
| ETF23 | 95 | 15 | 10 | 3 | 120.3 ± 5.92 | −52.5 ± 5.23 | 58.73 ± 4.15 | 41.35 ± 1.26 |
| ETF24 | 95 | 15 | 5 | 1.75 | 91.5 ± 6.12 | −42.5 ± 4.20 | 51.15 ± 1.70 | 17.14 ± 2.44 |
| ETF25 | 85 | 5 | 10 | 3 | 113.5 ± 4.58 | −46.1 ± 5.56 | 56.89 ± 2.80 | 26.00 ± 1.50 |
| ETF26 | 75 | 5 | 10 | 1.75 | 135.8 ± 6.74 | −47.1 ± 4.85 | 42.21 ± 2.34 | 9.00 ± 1.180 |
| ETF27 | 75 | 15 | 15 | 1.75 | 159.2 ± 7.83 | −54.1 ± 4.84 | 59.50 ± 1.50 | 24.80 ± 3.28 |
| ETF28 | 85 | 25 | 10 | 0.5 | 109.5 ± 4.37 | −58.1 ± 4.98 | 47.36 ± 3.60 | 25.00 ± 2.27 |
| ETF29 | 85 | 15 | 10 | 1.75 | 108.5 ± 3.61 | −46.1 ± 4.15 | 73.89 ± 1.58 | 41.64 ± 1.58 |
| EGCG-TF | 85 | 15 | 10 | – | 96.5 ± 5.58 | −41.4 ± 3.56 | 70.58 ± 1.63 | – |
SPC: soy phosphatidyl choline; SC: sodium cholate; EGCG: epigallocatechin-3-gallate; HA: hyaluronic acid; PDI: polydispersity index; EE: entrapment efficiency.
Values are expressed as mean ± SD; n = 3.
a
Significantly influenced by independent variables (SPC, SC and EGCG) (p < 0.05).
b
Not significantly influenced by independent variables (SPC, SC, EGCG and HA) (p > 0.05).