Figure 4.

Citrate synthase activity and protein stability are not altered by NCT-503 treatment in neuroblastoma cells. (A) PHGDH knockout clones #11, #38 and the respective control cells were seeded, treated with 10 µM NCT-503 or inactive drug control for 24 h and assessed for citrate synthase activity. (B) Wildtype BE(2)-C cells were seeded, treated with 10 µM NCT-503 or inactive drug control for 48 h and subjected to cellular thermal shift assays followed by western blotting of PHGDH and citrate synthase expression. (C) Schematic model summarising the NCT-503 treatment-mediated effects in neuroblastoma cells.