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. 2021 Jul 1;10(1):1309–1319. doi: 10.1080/22221751.2021.1908098

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Overexpression of SLY but not mSLY in P1/7 causes hyperactivation of inflammasome. THP-1 cells were primed with LPS and then infected with S. suis strains SC-19, msly (P353L), P1/7-Vec, P1/7-SLY, P1/7-mSLY or stimulated with ouabain. (A) The cellular proteins and the supernatants of the cell cultures were collected for the detection of Casp1, IL-1β, and GSDMD by western blot assay. (B) The concentrations of LDH, IL-1β, and TNF-α in the supernatants of THP-1 cells were detected (n = 3).