Figure 6.

Differences in lipid content resulting from the action of AR and the variants. (A) Phase contrast images of Oil red O staining for lipid accumulation in LNCaP-AR^v567es cells treated with vehicle, 10 nM R1881, or 30 ng/mL Dox for 6 days in medium containing CSS. (B) Heat map representing Z scores of individual lipids whose level changes as a result of the action of AR, ARv7, and AR^v567es. LNCaP-ARv7 cells were treated with vehicle (0.1% ethanol), 10 nM R1881 or 20 ng/mL Dox along with LNCaP-AR^v567es cells that were treated with 30 ng/mL Dox for 48 hours in CSS medium. Four biological replicates collected from 4 independent experiments were used. Five million cells from each treatment were subjected to liquid chromatography and mass spectrometry to identify the lipids.