FIGURE 3.

5‐Aminolevulinic acid (5‐ALA)/sodium ferrous citrate (SFC) increased tumor‐infiltrating lymphocyte (TIL) proliferation, and the expression levels of cytokines and cytolytic enzymes. Representative FACS data of the gated population. Intracellular staining of Ki‐67, interleukin‐2 (IL‐2), γ‐interferon (IFN‐γ), and Granzyme B (GrB) (left) and the quantified data (right) of TILs from B16F10‐bearing mice. Data represent the mean ± SD. *P < .05 by one‐way ANOVA with Tukey’s multiple comparisons test. Δmean fluorescent intensity (MFI) ＝ MFI− fluorescence minus one (FMO) MFI