FIGURE 4.

5‐Aminolevulinic acid (5‐ALA)/sodium ferrous citrate (SFC) enhanced the mitochondrial function by oxidative phosphorylation. A, Stimulated spleen cells were treated with or without 5‐ALA/SFC (1 mmol/L/0.5 mmol/L) for 72 h, and then the expression levels of complex I‐V proteins were detected by immunoblotting and analyzed by a one‐tailed unpaired Student’s t test. B, Enzymatic activity of Complex IV in stimulated spleen cells with or without 5‐ALA/SFC was detected by microplate assay kits was analyzed by a one‐tailed Wilcoxon matched‐pairs signed rank test. C, ATP levels in stimulated spleen cells with or without 5‐ALA/SFC measured by an ATP assay kit were analyzed by a one‐tailed unpaired Student’s t test. Each dot represents one independent experiment. Data represent the mean ± SD. *P < .05