FIGURE 2.

Loss of Par3 promotes bladder cancer (BLCA) metastasis. A, Par3 does not affect the proliferation of BLCA cells. CCK‐8 cell proliferation assays were performed with the BLCA stable cells mentioned in Figure S2B‐C. Data are presented as mean ± SD. B‐E, Par3 deficiency promotes the migration and invasion of BLCA cells. Transwell assays were used to determine the migration (B, D; left panel) and invasion (C, E; left panel) abilities of the referred BLCA stable cells. Scale bar, 100 μm. Quantification of the relative cells (B‐E, left panel) was performed by ImageJ and is shown in the right panels of B‐E, respectively. F‐G, Par3 deficiency promotes lung metastasis of BLCA cells in vivo. Lung metastatic tumor models were developed by injecting indicated BLCA cells into the tail vein of NOD‐SCID mice. Hematoxylin‐eosin (HE) staining images of lung metastatic nodules from each group were exhibited (F, G; left panel). Left scale bar, 200 μm; right scale bar, 50 μm. Ten regions were randomly selected from each mouse lung, and the number of metastatic nodes were counted (F, G; right panel). Values are mean ± SD. n = 6. ***P <.001 by Student's t‐test