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. 2021 May 10;112(7):2855–2869. doi: 10.1111/cas.14897

FIGURE 6.

FIGURE 6

TET1‐targeted gene, HMGA2, enhances hepatoma cell proliferation. A, HMGA2 expression of HepG2 and Huh7 cells with siCTL, HMGA2 siRNA (siHMGA2‐1 and ‐2), and siTET1. Left, relative HMGA2 mRNA expression levels by RT‐qPCR compared with cells with siCTL. Right, HMGA2 expression by Western blotting analysis using anti‐HMGA2 antibody and anti‐β‐actin antibody. B, The tumor proliferation curves of HepG2 and Huh7 cells with siCTL and siHMGA2 by WST‐8. C, HMGA2 expression of HepG2 and Huh7 cells with control (LacZ) and HMGA2 overexpressed (HMGA2) by pLenti6.3/V5 vectors. Upper, relative HMGA2 mRNA expressions by RT‐qPCR compared with cells with LacZ. Lower, HMGA2 expression by Western blotting analysis using anti‐HMGA2 antibody. Arrow and arrowhead indicate the overexpressed HMGA2 proteins and endogenous HMGA2 proteins, respectively. D, The tumor proliferation curves of HepG2 and Huh7 cells with siCTL, siTET1, LacZ, and HMGA2 by WST‐8. Data are shown as the mean ± SD from triplicate experiments in (B) and (D). P‐values were measured using Student's t‐test. *P < .05; **P < .01; ***P < .001. E, Transcriptional regulation of oncogenic target HMGA2 through enhancer cytosine hydroxymethylation, histone modification, and chromatin interaction