FIGURE 1.

Knockdown of HSP47 dampens the chemoresistance of pancreatic cancer cells. A, Expression of HSP47 mRNA in PDAC cells (PANC1 cells, MIA‐PaCa2 cells, and Suit2 cells). B, Expression of HSP47 protein in PDAC cells. C, Expression of HSP47 mRNA in PDAC cells transfected with control siRNA (siControl, 10 nmol/L) and 2 batches (A and B) of HSP47 siRNA (siHSP47, 10 nmol/L). D, Expression of HSP47 protein in PDAC cells transfected with siControl, siHSP47‐A, and siHSP47‐B. E, In vitro proliferation of PDAC cells transfected with siControl, siHSP47‐A, and siHSP47‐B. F, Viability of HSP47‐silenced PDAC cells after treatment with gemcitabine at each indicated concentration. G, IC₅₀ values of HSP47‐silenced PDAC cells after treatment with gemcitabine at each indicated concentration. *, P < .05. n.s., not significant