FIGURE 6.

HSP47 alleviates the ROS‐induced increase in intracellular Ca²⁺ levels in pancreatic cancer cells after treatment with gemcitabine. A, Levels of intracellular Ca²⁺ in HSP47 KO PDAC cells (PANC1 cells) after treatment with NAC (10 μmol/L), DPI (2 μmol/L) and gemcitabine. Intracellular Ca²⁺ levels were determined using flow cytometry and calculating the intensity of Fluo‐4. B, Levels of intracellular Ca²⁺ in Mock:PDAC cells (PANC1 cells and MIA‐PaCa2 cells), HSP47 KO PDAC cells and HSP47 KO PDAC cells expressing HSP47‐Myc at 24 h after treatment with H₂O₂ (2 μg/mL). C, Changes in intracellular Ca²⁺ levels in Mock:PDAC cells, HSP47 KO PDAC cells, and HSP47 KO PDAC cells expressing HSP47‐Myc after treatment with H₂O₂. Changes in intracellular Ca²⁺ levels were determined by calculating the ratio (F340/F380). D, Changes in intracellular Ca²⁺ levels in Mock:PANC1 cells, CALR KO PANC1 cells, HSP47 KO PANC1 cells and CALR KO HSP47 KO PANC1 cells after treatment with H₂O₂ (2 μg/mL). E, Changes in intracellular Ca²⁺ levels in Mock:PANC1 cells, IRE1α KO PANC1 cells, HSP47 KO PANC1 cells and IRE1α KO HSP47 KO PANC1 cells after treatment with H₂O₂ (2 μg/mL). * P < .05. n.s: not significant