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. 2021 Jul 2;16(7):e0254000. doi: 10.1371/journal.pone.0254000

Table 4. IHC expression of EMT-related markers vs. cancer stages classified into 0 to IV.

Cancer Stage
0 p-value
E-cadherin 0.201
+1 0 (0.00) 0 (0.00) 0 (0.00) 4 (7.27) 1 (3.57)
+2 0 (0.00) 3 (18.75) 3 (7.32) 8 (14.55) 8 (28.57)
+3 2 (100.00) 13 (81.25) 38 (92.68) 43 (78.18) 19 (67.86)
β-catenin 0.951
0 1 (50.00) 9 (56.25) 17 (41.46) 22 (40.74) 15 (53.57)
+1 1 (50.00) 5 (31.25) 14 (34.15) 15 (27.78) 7 (25.00)
+2 0 (0.00) 2 (12.50) 8 (19.51) 15 (27.78) 5 (17.86)
+3 0 (0.00) 0 (0.00) 2 (4.88) 2 (3.70) 1 (3.57)
Claudin-1 0.293
0 1 (50.00) 1 (6.25) 0 (0.00) 7 (12.73) 3 (10.71)
+1 1 (50.00) 7 (43.75) 15 (36.59) 16 (29.09) 10 (35.71)
+2 0 (0.00) 7 (43.75) 19 (46.34) 25 (45.45) 9 (32.14)
+3 0 (0.00) 1 (6.25) 7 (17.07) 7 (12.73) 6 (21.43)
CD44 0.646
0 0 (0.00) 2 (12.50) 1 (2.44) 5 (9.26) 2 (7.14)
+1 1 (50.00) 5 (31.25) 10 (24.39) 16 (29.63) 13 (46.43)
+2 1 (50.00) 5 (31.25) 21 (51.22) 24 (44.44) 11 (39.29)
+3 0 (0.00) 4 (25.00) 9 (21.95) 9 (16.67) 2 (7.14)
N-cadherin 0.350
0 2 (100.00) 16 (100.00) 41 (100.00) 52 (94.55) 27 (96.43)
+1 0 (0.00) 0 (0.00) 0 (0.00) 3 (5.45) 0 (0.00)
+2 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 1 (3.57)
Fibronectin 0.291
0 1 (50.00) 2 (12.50) 2 (4.88) 6 (10.91) 1 (3.57)
+1 0 (0.00) 11 (68.75) 20 (48.78) 33 (60.00) 20 (71.43)
+2 1 (50.00) 3 (18.75) 18 (43.90) 15 (27.27) 6 (21.43)
+3 0 (0.00) 0 (0.00) 1 (2.44) 1 (1.82) 1 (3.57)
Vimentin 0.393
negative 2 (100.00) 16 (100.00) 41 (100.00) 55 (100.00) 27 (96.43)
positive 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 1 (3.57)
S100A4 0.208
0 1 (50.00) 10 (62.50) 7 (17.07) 13 (23.64) 7 (25.00)
+1 1 (50.00) 3 (18.75) 21 (51.22) 27 (49.90) 10 (35.71)
+2 0 (0.00) 2 (12.50) 8 (19.51) 9 (16.36) 7 (25.00)
+3 0 (0.00) 1 (6.25) 5 (12.20) 6 (10.91) 4 (14.29)