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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochimie. 2020 Oct 24;180:1–9. doi: 10.1016/j.biochi.2020.10.012

FIGURE 3. Snail promotes cancer cell adhesion to nerve cells in vitro.

FIGURE 3.

NS20Y nerve cells were plated in a 96 well plate and incubated overnight. LNCaP Neo (empty vector) or LNCaP Snail 18 (clone overexpressing Snail) and C4-2 NS (control) or C4-2 Snail shRNA (knockdown) were cultured with nerve cells for 30 minutes, and cells which were attached were fixed with percoll fixative, stained with crystal violet. The cancer cells were then counted; they are easily identified by their extended morphology, since the NS20Y remain small and rounded before prolonged stimulation. Cancer adhesion levels quantitated are representative of triplicate experiments done twice independently (n = 6, *p< 0.05, ****p< 0.0001; ns, non-significant). Statistical analysis was done using GraphPad Prism.