Table 1.
Comparison of the infection and silencing features of BMVCP5 containing different TaPDS inserts.
| VIGS vector | Infection frequency (%)a | Silencing frequency (%)b | Number of plants with bleached leaf | |||
|---|---|---|---|---|---|---|
| L2c | L3c | L4c | L5c | |||
| BMV:TaPDS100 | 76.9 (10/13) | 100.0 (10/10) | 10 | 10 | 10 | 10 |
| BMV:TaPDS150 | 88.9 (8/9) | 75.0 (6/8) | 4 | 6 | 6 | 0 |
| BMV:TaPDS200 | 72.7 (8/11) | 87.5 (7/8) | 7 | 7 | 5 | 0 |
| BMV:TaPDS250 | 66.7 (8/12) | 87.5 (7/8) | 0 | 5 | 7 | 0 |
| BMV:eGFP180 | 91.7 (11/12) | 0.0 (0/11) | 0 | 0 | 0 | 0 |
Coleoptiles of 3 day-old wheat seedlings (cv. Overley) were rub-inoculated with sap from N. benthamiana leaves infiltrated with BMV VIGS vectors. Phenotypic data collected at 28 dpi.
a
Calculated by dividing the number of plants infected by the total plants inoculated.
b
Calculated by dividing the number of plants showing bleaching phenotype by the total plants infected.
c
Systemically infected leaf (L) designated from bottom to top (2nd to 5th leaf above coleoptile).