| Cell line (human) |
HCT116 PABPC1-AID (sC152-C16) |
This study |
sC152-C16 |
See Materials and methods for details |
| Cell line (human) |
HCT116 PABPC1-AID (sC278-C2) |
This study |
sC278-C2 |
See Materials and methods for details |
| Cell line (human) |
HCT116 OsTIR |
Natsume et al., 2016 |
|
|
| Cell line (human) |
HeLa |
Nam et al., 2014 |
|
|
| Cell line (human) |
HeLa RPL3-3xHA (sC262-4) |
This study |
sC262-4 |
See Materials and methods for details |
| Cell line (mouse) |
NIH3T3 |
Eisen et al., 2020 |
|
|
| Cell line (zebrafish) |
ZF4 |
ATCC (CRL-2050) |
|
|
| Antibody |
Anti-β-actin (Rabbit monoclonal) |
Cell Signalling Technology (D6A8) |
|
1:1000 dilution |
| Antibody |
Anti-AID (Mouse monoclonal) |
MBL International (M214-3) |
|
1:1000 dilution |
| Antibody |
Anti-ePAB (Rabbit polyclonal) |
Wilkie et al., 2005 |
|
1:2000 dilution |
| Antibody |
Anti-GFP (Mouse monoclonal) |
Cell Signalling Technology (2955) |
|
1:1000 dilution |
| Antibody |
Anti-FLAG (Mouse monoclonal) |
MilliporeSigma (F9291) |
|
1:1000 dilution |
| Antibody |
Anti-GAPDH (Mouse monoclonal) |
Proteintech (60004) |
|
1:1000 dilution |
| Antibody |
Anti-HA (Mouse monoclonal) |
Cell Signalling Technology (6E2) |
|
1:1000 dilution |
| Antibody |
Anti-PABPC1 (Rabbit polyclonal) |
Cell Signalling Technology (4992S) |
|
1:1000 dilution |
| Antibody |
Anti-PABPC4 (Rabbit polyclonal) |
Novus Biologicals (NB100-74594) |
|
1:1000 dilution |
| Antibody |
Anti-puromycin (Mouse monoclonal) |
MilliporeSigma (MABE343) |
|
1:1000 dilution |
| Antibody |
Anti-RPL3 (Rabbit polyclonal) |
Abcam (ab154882) |
|
1:1000 dilution |
| Antibody |
Anti-RPS15 (Rabbit polyclonal) |
Proteintech (14957–1-AP) |
|
1:1000 dilution |
| Antibody |
Anti-RPS3 (Rabbit monoclonal) |
Cell Signalling Technology (D50G7) |
|
1:1000 dilution |
| Antibody |
Anti-TUT4 (Rabbit polyclonal) |
ABclonal (A5972) |
|
1:1000 dilution |
| Antibody |
Anti-TUT7 (Rabbit polyclonal) |
Proteintech (25196–1-AP) |
|
1:1000 dilution |
| Antibody |
Anti-Vinculin (Mouse monoclonal) |
Proteintech (66305) |
|
1:1000 dilution |
| Antibody |
IRDye 800CW Goat anti-Rabbit IgG (H + L) |
LI-COR (926–32211) |
|
1:10,000 dilution |
| Antibody |
IRDye 680RD Goat anti-Mouse IgG |
LI-COR (926–68070) |
|
1:10,000 dilution |
| Sequence based reagent |
All oligos |
Supplementary file 1 |
|
|
| Recombinant DNA reagent |
All plasmids |
Supplementary file 2 |
|
|
| Other |
Reference sequences |
Supplementary file 3 |
|
|
| Other |
Masked mitochondrial pseudogenes |
Supplementary file 4 |
|
|
| Other |
mRNA 3′-end annotations |
Supplementary file 5 |
|
|
