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Effects of circ_0044516 siRNA on lung cancer stem cell properties. (a, b) Spheroid formation assays were carried out using A549 and SPCA1 cells transfected with circ_0044516 siRNA or NC. (c, d) Flow cytometric assays were used to evaluate the CD133-positive cell ratio in A549 and SPCA1 cells. (e, f) Sox2, Nanog, Oct4, and CD133 mRNA expression. Data are presented as means ± SD. ∗P < 0.05.
