Figure 5.

miR-136 is a direct target of circ_0044516. (a) miR-136 expression in 20 pairs of lung cancer tissues. (b) RNA pull-down assays were carried out using biotin-miR-136, control, or 10% input. (c) Predicted WT or MUT miR-136 binding sites in circ_0044516. (d) Relative luciferase activities were measured in A549 and SPCA1 cells cotransfected with circ_0044516-WT or circ_0044516-MUT and miR-136 mimics or miR-NC by luciferase reporter gene assays. (e) Expression of miR-136 in lung cancer cells transfected with circ_0044516 siRNA or circ_0044516 overexpression plasmid by RT-qPCR. Data are presented as means ± SD. ^∗P < 0.05; ^∗∗∗P < 0.001.