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. 2021 Jun 24;2021:5510869. doi: 10.1155/2021/5510869

Figure 6.

Figure 6

MAT2A is a direct target of miR-136. (a) MAT2A mRNA expression in 20 pairs of lung cancer tissues. (b) RNA pull-down assays were carried out with biotin-miR-136, control, or 10% input using A549 or SPCA1 cell extracts. (c) Predicted WT and MUT miR-136 binding sites in MAT2A. (d) Relative luciferase activities were measured in A549 and SPCA1 cells cotransfected with MAT2A-WT or MAT2A-MUT and miR-136 mimics or miR-NC by luciferase reporter gene assays. (e) Expression of MAT2A in lung cancer cells transfected with miR-136 mimics or miR-136 mimics combined with circ_0044516 overexpression plasmid. Data are presented as means ± SD. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.