Figure 7.

Circ_0044516 induced lung cancer cell proliferation, invasion, and cancer stem cell characteristics by modulating the miR-136/MAT2A pathway. (a, b) Expression of MAT2A in A549 and SPCA1 cells transfected with corresponding plasmids. (c, d) Cell proliferation of A549 and SPCA1 cells. (e) Cell invasion by A549 cells. (f) Tumor spheroid formation. Twelve 5-week old female BALB/c nude mice were injected with A549 cells infected with circ_0044516 siRNA (six mice) or NC (six mice). (g) Tumor volume. (h) Tumor weight. (i) Expression of circ_0044516, miR-136, and MAT2A. (j) Protein expression of MAT2A. Data are presented as means ± SD. ^∗P < 0.05.