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. 2021 Jul 2;4:835. doi: 10.1038/s42003-021-02342-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Visualization and BSP (Bisulfite-Sequencing PCR) analysis of selected putative spaceflight-induced heritable DMRs and expression levels of a LHCA1, b TGA4, and c TGA1 in the offspring of spaceflight and ground control plants. Visualization of methylation data was performed, using the Integrated Genome Viewer (IGV) (http://software.roadinstitute.org/software/igv). The inherited methylation level of CpG islands was verified by using BSP analysis. The error bar is the standard deviation. An asterisk * indicates a statistically significant difference between the spaceflight and control samples using Student’s two-tailed t-test (p < 0.05).