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. 2021 Jul 2;7:85. doi: 10.1038/s41523-021-00290-0

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Brightfield cytology imaging of non-tumor human breast tissue specimens were sampled by FNA and imaged by standard benchtop microscopy (a) and brightfield imaging by EpiView (b). Left panels: LPF views by standard microscope equipped with a 10× objective lens (top) and native view on EpiView (bottom). Right panels: standard microscope image obtained by a 40× objective (top), showing the same ROI outlined with red dashed line in the 10× image, and a similar view obtained by digital zoom on the EpiView (bottom). Scale bars for LPF and HPF images are 0.2 mm and 30 µm, respectively. c, d Similar sequence of images as a and b but with a representative specimen with cytological evidence of tumor. e Results of HER2 credentialing by EpiView-D4 for 19 different patient breast tissue specimens: non-tumor, HER2-negative tumor, HER2-positive tumor (N = 7, 6, 6, respectively). Each data point represents the mean D4 fluorescence intensity (after background subtraction) of duplicate assays measured by the EpiView-D4 for an individual tissue specimen. The horizontal line represents the mean D4 signal intensity for each category. There was a statistically significant difference between groups as determined by one-way ANOVA (F (2, 16) = 9.264, p = 0.0021). Groups that are different by multiple comparison testing (Tukey post hoc test, p ≤ 0.05) are marked with different letters. HER2-positive tumors are defined as IHC 3+ or IHC 2+ with HER2 amplification by FISH. f EpiView-D4 readout vs ELISA for HER2 for all patient specimens. A strong, positive correlation between EpiView-D4 and ELISA across the 19 paired measurements is observed, which was statistically significant (Pearson r = 0.956, p < 0.0001, 95% CI: 0.886–0.983).