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. 2021 Jul 2;11:13723. doi: 10.1038/s41598-021-93222-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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One to one comparison of PARS histological imaging and H&E staining. (a) Large field of view (9 mm × 13.25 mm) PARS (780 megapixel) image of nuclear morphology in a thin section of formalin fixed paraffin embedded excised breast tumor tissues. (b–d) (i) Subsections of the large field of view PARS image (a) highlighting regions of interest within the large field scan. (ii) Images of the exact same set of tissues following H&E staining, providing a direct one-to-one comparison between PARS imaging of tissues and the gold standard for histopathology H&E imaging. (a) Scale Bar: 2 mm. (b) Scale Bar: 200 µm. (c) Scale Bar: 50 µm. (d) Scale Bar: 75 µm.