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PARS 3D volumetric imaging of subcellular nuclear morphology in unprocessed excised Rattus mammary tumor tissue. (a) A subset of the optically sectioned volumetric image stack showing subsurface nuclear morphology from the surface to 30 µm into the tissue. Scale Bar: 125 µm. (b–d) Different angular views of the 3D reconstruction of the nuclei within the excised tissue sample. Scale Bar: 200 µm.
