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. 2021 Jul 2;11:13754. doi: 10.1038/s41598-021-93150-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IFN-γ production by PBMCs from healthy donors is reduced by incubation with either CBED or AMED eye drop solutions. (a) Results show percentage of maximum IFN-γ expression in culture supernatants relative to complete media following 2-h stimulation with PMA and ionomycin. Eye drop preparations investigated were CBED (n = 10) and AMED (n = 10). PBMCs were cultured with 50% eye drop solution (diluted with complete media and IL-2) or complete media and IL-2 only, for 48 h prior to PMA and ionomycin stimulation. Each experiment was repeated with four different PBMC donors and data points represent donor means. (b) ELISA quantification of soluble NKG2D ligands (sMICA, sMICB and sULBP1) in CBED in pg/mL. Statistical analysis was performed using nonparametric Mann–Whitney test. p < 0.0001 (^****).