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. 2021 Jul 2;11:13766. doi: 10.1038/s41598-021-93216-6

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of true hermaphrodites from the B6.Y^TIR cross. (a) Distribution of genotypes and sex phenotypes among the offspring of XY^TIR males. (b–d) Male (XY.MT) and hermaphrodite (XY.HT) phenotypes. (b) Different anogenital distance and mammary gland development in males and hermaphrodites (arrowheads point to mammary glands); (c) Males show variable testes weight with either two testes of similar weight (top row) or one testis larger than the other (middle row). Hermaphrodites have unilateral small testis and contralateral ovary (XY.HT OV + T) (bottom row) or a streak gonad (XY.HT S + T) (not shown); (d) Testis weight in males and hermaphrodites. Error bars show standard deviation. Asterisk indicates significant difference *P < 0.05 (one-tailed t-test).