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. 2021 Jul 2;4:832. doi: 10.1038/s42003-021-02295-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a FACS sorting of CD15s^high and CD15s^low fraction of CD15s^bimodal individuals. FACS profile and sorting gates of the CD15s^low and CD15s^high basophil fraction are shown for one representative donor from six donors. b Volcano plot. The plot summarized the edgeR paired RNA-seq results of the pairs of CD15s^high vs. CD15s^low fractions obtained from six CD15s^bimodal donors. Each dot represents a gene and the plot displays the fold change (log2) vs. nominal p value (−log10). Boundaries for nominal and adjusted p value are indicated. c Validation by qPCR. The mRNA expression of FUT6 (n = 8) and FUT7 (n = 6) in the CD15s^low and CD15s^high fraction validated by qPCR. Paired samples are connected by lines; p values are indicated. Statistical comparison was performed using Wilcoxon matched-pairs signed-rank test.