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. 2021 Jul 2;4:832. doi: 10.1038/s42003-021-02295-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a FUT6 transcripts in basophils from the CD15s^high and CD15s^low fraction. cDNAs from the CD15s^high and CD15s^low basophil fraction of two CD15s^bimodal individuals were isolated, and the region encoding for FUT6 amplified by PCR and sequenced. The upper panel displays the FUT6 sequence of the genomic DNA and the two cDNA regions covering three coding SNPs (rs61147939, rs145035679, and rs61739552). Each of the donors was heterozygous for two of these SNPs. Polymorphic bases are highlighted in red; base pair position as well as encoded amino acid residues are indicated. The histograms of the sequencing are displayed in the lower panels. Signals derived from polymorphic sites are indicated by red arrows. b ST3GAL1 transcripts. As a control, cDNA sequencing of ST3GAL1, a gene not known to be controlled by random monoallelic expression, is shown. The donor was heterozygous for rs1048479. Only the sequence proximal to this SNP is shown.