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. 2021 Jul 2;12:4105. doi: 10.1038/s41467-021-24352-w

Fig. 4. Enhanced intradermal IL-6 alleviates psoriasiform skin inflammation.

Fig. 4

a–c Zc3h12c, Tnf, and Il6 expression in imiquimod (IMQ)-treated skin of WT mice at indicated days was measured by qPCR (a: n = 4 mice for day 0 and 3, n = 3 mice for day 1 and 6; b: n = 4 mice for day 0, n = 3 mice for day 1, 3, and 6; c: n = 3 mice for day 0, 1, and 3, n = 4 mice for day 6). d TNF secretion by IMQ-treated skin of Zc3h12c+/+(blue)/Zc3h12c−/− (red)mice at day 2 was measured by ELISA after normalizing with weight (n = 5 mice per group). e Il12p40 expression of IMQ-treated skin of Zc3h12c+/+/Zc3h12c−/− mice at day 3 was measured by qPCR (n = 4 mice per group). f IL-6 secretion by IMQ-treated skin of Zc3h12c+/+/Zc3h12c−/− mice at day 3 was measured by ELISA after normalizing with weight (n = 3 mice per group). g, h TNF production by infiltrated myeloid cells from Zc3h12c+/+/Zc3h12c-−/− IMQ-treated skin on day 3 was measured by intracellular staining after R848 activation (g: n = 8 mice for Zc3h12c+/+; n = 9 mice for Zc3h12c−/−; h: n = 5 mice per group). i TNF production by infiltrated macrophages (CD45+ CD11b+ F4/80+ CD64+) from Zc3h12cfl/fl/Zc3h12cMΦΔ IMQ-treated skin on day 4 was measured by intracellular staining after R848 activation (n = 9 mice per group). j TNF production by infiltrated macrophages (CD45+ CD11c− CD11b+ F4/80+ CD64+) from Zc3h12cfl/fl/ Zc3h12cDCΔ IMQ-treated skin on day 4 was measured by intracellular staining after R848 activation (n = 4 mice per group). k Psoriasis severity of IMQ-treated WT mice with rIL-6 injections were scored by mPASI daily (n = 5 mice per group). l TNF secretion by IMQ + rIL-6-treated skin at day 3 was measured by ELISA after normalizing with weight (n = 5 mice per group). m Il17a expression in IMQ + rIL-6 treated skin of WT mice at indicated days was measured by qPCR (n = 5 mice per group). n IL-6 secretion by treated skin was measured by ELISA after normalizing with weight (n = 4 mice per group). o Zc3h12c expression in UV-treated skin at 24 h was measured by qPCR (n = 5 mice per group). p Zc3h12c expression in IMQ + rIL-6-treated skin at day 4 was measured by qPCR (n = 5 mice per group). Data are representative of at least three independent experiments (mean ± S.D.; two-tailed Student’s t test). Source data are provided as a Source Data file.