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. 2021 Jul 2;12:4105. doi: 10.1038/s41467-021-24352-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Psoriasis severity of imiquimod (IMQ)-treated skin from Zc3h12c^+/+(blue)/Zc3h12c^−/− (red)mice was measured daily by mPASI (n = 4 mice per group). Representative pictures of the treated back-skin were shown at right. b–d Percentages of infiltrated immune cells from IMQ-treated Zc3h12c^+/+/Zc3h12c^−/− back-skin on day 3 were analyzed by flow cytometry (n = 4 mice for Zc3h12c^+/+; n = 5 mice for Zc3h12c^−/−). Cells were live gated as following: CD45⁺ leukocytes (Leu), CD45⁺ CD11b⁺ F4/80⁺ CD64⁺ macrophages (MΦ), CD45⁺ CD11b⁺ Ly6G⁺ neutrophils (Neu), CD45⁺ CD11b⁺ Ly6C⁺ monocytes (Mono). e, f IL-17A production by CD45⁺ leukocytes (e) and CD3ε⁺ γδTCR⁺ T cells (f) at Zc3h12c^+/+/Zc3h12c^−/− IMQ-treated skin on day 3 was measured by intracellular staining (n = 4 mice for Zc3h12c^+/+; n = 5 mice for Zc3h12c^−/−). g γδ T-cell subset composition at IMQ-treated Zc3h12c^+/+/Zc3h12c^−/− back-skin on day 3 was analyzed by flow cytometry (n = 4 mice for Zc3h12c^+/+; n = 5 mice for Zc3h12c^−/−). Dermal (γδTCR⁺ TCRβ⁻) and epithelial (γδTCR⁺ TCRβ⁺) γδ T-cell subsets were gated from live⁺ CD45⁺ CD3ε⁺. h–k Psoriasis severity of IMQ-treated Zc3h12c^fl/fl/Zc3h12c^MΦΔ/Zc3h12c^DCΔ mice were scored by mPASI daily (h, j), and IL-17A production by γδ T cells (i, k) at day 4 was measured (n = 4 mice per group). l Skin thickness of rIL-23-treated ears of Zc3h12c^+/+/Zc3h12c^−/− mice was measured daily (n = 5 mice for Zc3h12c^+/+; n = 4 mice for Zc3h12c^−/−). m TNF production by infiltrated macrophages rIL-23-treated ears of Zc3h12c^+/+/Zc3h12c^−/− mice at day 9 (n = 5 mice for Zc3h12c^+/+; n = 4 mice for Zc3h12c^−/−). n IL-17A production by γδ T cells at rIL-23-treated ear on day 9 was measured by intracellular staining (n = 5 mice for Zc3h12c^+/+; n = 4 mice for Zc3h12c^−/−). o EAE score of Zc3h12c^+/+/Zc3h12c^−/− mice (n = 5 mice per group). p Psoriasis severity of the chimeras were scored by mPASI daily (n = 5 mice per group). q TNF production by infiltrated macrophages from the chimeras at day 4 was measured by intracellular staining after R848 activation (n = 5 mice per group). Data are representative of five (a–g), or two (h–q) independent experiments (mean ± S.D.; two-tailed Student’s t test). Source data are provided as a Source Data file.